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在已掌握甘蓝型油菜-萝卜附加系染色体构成,并构建萝卜分子连锁图谱的基础上,以甘蓝型油菜?萝卜附加系为材料,利用(dp)RAPD分子标记技术,筛选附加系中外源萝卜9条染色体(A～I)的分子标记,为进一步研究萝卜染色体与连锁群对应关系奠定基础。试验筛选了576个随机引物(组合),有413个能从附加系中扩增出萝卜染色体的(dp)RAPD标记,平均有效引物(组合)占71·7%,其中RAPD反应有效引物率67·7%,dpRAPD反应有效引物率73·2%。试验共得到899个萝卜染色体(dp)RAPD标记,包括354个RAPD标记和545个dpRAPD标记,覆盖萝卜整个基因组的全部9条染色体,不同染色体的标记数目变化范围在41～160,其中最多的是染色体C,最少的是染色体I。
Based on the chromosomal structure of the additional lines of Brassica napus and radish and the molecular linkage map of radish, the RAPD marker (RAPD) technique was used to detect the exogenous radish 9 The molecular markers of chromosomes (A ~ I) laid the foundation for further study on the relationship between radish chromosomes and linkage groups. A total of 576 random primers (combinations) were screened and 413 (dp) RAPD markers were obtained. The average effective primer (combination) accounted for 71.7% of the total, and the effective rate of RAPD primer 67 · 7%, dpRAPD reaction effective primer rate of 73.2%. A total of 899 radish chromosome (dp) RAPD markers were obtained, including 354 RAPD markers and 545 dpRAPD markers covering all nine chromosomes of the whole radish genome. The number of markers on different chromosomes ranged from 41 to 160, most of which Chromosome C, the least chromosome I.