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本文用放免法对564例慢性乙型肝炎及肝炎后肝硬变患者血清的HBV标志物进行检测,并对HBV的特殊血清类型从临床角度进行分析探讨。对象与方法一、对象:564例慢性肝炎及肝硬变患者均为我院传染科病人,肝炎病史及血清HBV标志物阳性均在一年以上,均抽血5mi,收集全部血清—20℃保存,最后一次性检测。二、血清HBV标志物(HBVM):HBsAg、HBsAg—IgM、抗HBs、HBeAg、抗HBe、HBcA—g、抗HBc、抗HBc—IgM、PHSAR、Pre—S2等10项,均用固相放免法,试剂购自山东3V公司,仪器采用中国科技大学GC—911放射免疫伽玛计数器。
In this paper, 564 patients with chronic hepatitis B and post-hepatitis cirrhosis serum HBV markers were detected by radioimmunoassay, and the specific serum types of HBV were analyzed from the clinical point of view. Subjects and methods First, the object: 564 patients with chronic hepatitis and cirrhosis were infected patients in our hospital, hepatitis history and serum HBV markers were positive for more than one year, both blood 5mi, collect all serum -20 ℃ save , Last one-time test. Serum HBV markers: HBsAg, HBsAg-IgM, anti-HBs, HBeAg, anti-HBe, HBcA-g, anti-HBc, anti-HBc-IgM, PHSAR, Pre- France, reagents purchased from Shandong 3V company, the instrument uses the China University of Technology GC-911 radioimmuno gamma counter.