论文部分内容阅读
目的:建立以高效液相色谱法同时测定复方盐酸利多卡因洗液中3组分含量的方法。方法:色谱柱为C18,流动相为甲醇-水-三乙胺(58∶42∶0.4),用磷酸调pH至3.0,流速为1mL.min-1,检测波长为240nm,进样量为20μL,柱温为22℃~28℃。结果:利多卡因、醋酸氯己定及曲安奈德检测浓度的线性范围分别为369.6~985.6(r=0.9998)、84.0~224.0(r=0.9998)、12.0~32.0μg.mL-1(r=0.9999);平均回收率分别为99.58%(RSD=0.41%)、99.51%(RSD=0.83%)、100.00%(RSD=0.68%)。结论:本方法操作简便、快速、准确、可靠,可用于该制剂的质量控制。
Objective: To establish a method for simultaneous determination of 3 components in compound lidocaine hydrochloride lotion by high performance liquid chromatography. Methods: The column was C18, the mobile phase was methanol-water-triethylamine (58:42:0.4), adjusted to pH 3.0 with phosphoric acid, the flow rate was 1mL.min-1, the detection wavelength was 240nm and the injection volume was 20μL The column temperature was 22 ℃ ~ 28 ℃. Results: The linear ranges of lidocaine, chlorhexidine acetate and triamcinolone acetonide were 369.6-985.6 (r = 0.9998), 84.0-224.0 (r = 0.9998), 12.0-32.0μg.mL-1 (r = 0.9999). The average recoveries were 99.58% (RSD = 0.41%), 99.51% (RSD = 0.83%) and 100.00% (RSD = 0.68%), respectively. Conclusion: The method is simple, rapid, accurate and reliable and can be used for the quality control of this preparation.