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利用筛选到的25个微卫星标记,以中华鳖极大、极小两个群体共120个个体为实验材料,分析这些微卫星标记与体重、背甲长、背甲宽、体高、腹甲长及腹甲宽之间的相关性。结果表明:有3个位点与生长性状的相关性达到显著或极显著水平(p<0.05或p<0.01),其中位点LTF1与体重、背甲长、背甲宽、腹甲长、腹甲宽、体高均极显著相关;位点LTR87与体重、背甲长、和腹甲宽显著相关;位点LT8与体重、体高、腹甲长、腹甲宽显著相关。将同一性状不同基因型之间进行多重比较,结果显示位点LTF1中的BE型,位点LTR87中的BB型,以及位点LT8中的CF型、BC型、AC型、CC型具有明显的生长优势,可作为辅助育种的参考标记。
Twenty-five microsatellite markers were screened from 120 individuals of two large and medium-sized Chinese soft-shelled turtles. The microsatellite markers were compared with body weight, carapace length, carapace width, body height, And the correlation between the width of the plastron. The results showed that the correlation between the three loci and the growth traits was significant or extremely significant (p <0.05 or p <0.01). The locus LTF1 was associated with body weight, carapace length, carapace width, A width and height were significantly correlated. Site LTR87 was significantly associated with body weight, carapace length and plastron width. Site LT8 was significantly associated with body weight, body height, plastron length and plastron width. Multiple comparisons between different genotypes of the same trait showed that BE locus locus LTF1, locus BB locus LTR87, locus LT8 locus CF locus BC locus AC locus CC locus Growth advantage, can be used as a reference marker breeding.