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采用离体孵育大鼠黄体细胞的方法，观察了反义c-fos寡脱氧核苷酸（反义c－fosODN）对hCG诱导的黄体细胞孕酮（P）和雌二醇（E2）产生的影响，同时观察了外源性cAMP和钙离子通道阻断剂维拉帕米（Verapamil）对黄体细胞中c－fos蛋白的影响。结果发现，反义c－fosODN能呈剂量相关方式抑制hCG诱导的黄体细胞P和E2的产生，同时使c－fos蛋白染色阳性的黄体细胞百分数下降；而无义tatODN没有相应的作用。10-4mol／L二丁酰cAMP能明显增强hCG对P、E2产生的刺激作用，并使c－fos蛋白染色阳性的黄体细胞百分率显著升高；当与反义c－fosODN共同作用时能明显逆转反义c-fosODN对P、E2产生和c－fos表达的抑制作用。10～6mol／L维拉帕米（Verapamil）显著抑制hCG诱导的P、E2产生，同时使c－fos蛋白染色阳性的黄体细胞百分率下降；当与反义c－fosODN共同作用时，对反义c-fosODN对P、E2产生和c－fos表达的抑制作用具有协同效应。表明，c－fos与hCG诱导的黄体细胞孕酮和雌二醇的产生密切相关；cAMP和Ca2+能调节c－fos在黄体细胞中的表达。
The effect of antisense c-fos oligodeoxynucleotides (antisense c-fosODN) on hCG-induced progesterone (P) and estradiol (E2) production of luteinized corpus luteum was studied by in vitro incubation of rat luteal cells. The effects of exogenous cAMP and the calcium channel blocker Verapamil on c-fos protein in luteal cells were also observed. The results showed that antisense c-fosODN could inhibit hCG-induced luteal P and E2 production in a dose-dependent manner, and at the same time, the percentage of luteal cells that were positive for c-fos protein staining was decreased. There was no corresponding effect of nonsense tatODN. The 10-4mol/L dibutyryl cAMP can significantly enhance the stimulation of hCG on P and E2 production, and significantly increase the percentage of luteal cells positive for c-fos protein staining; when combined with antisense c-fosODN, it can significantly Reverse the inhibitory effect of antisense c-fosODN on P, E2 production and c-fos expression. 10 to 6 mol/L verapamil significantly inhibited hCG-induced P and E2 production, while decreasing the percentage of luteal cells positive for c-fos protein staining; when co-acting with antisense c-fosODN, antisense c-fosODN has a synergistic effect on the inhibition of P, E2 production and c-fos expression. It was shown that c-fos is closely related to the production of progesterone and estradiol by luteinized cells induced by hCG; cAMP and Ca2+ can regulate the expression of c-fos in luteal cells.