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目的:建立四季感冒胶囊中橙皮苷和连翘苷的含量测定方法。方法:应用高效液相色谱法测定,色谱柱为AgilentXDB C18(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸,梯度洗脱,流速为1.0 ml·min-1,柱温为30℃,检测波长为277nm,进样量为5μl。结果:橙皮苷和连翘苷的线性范围分别为12.000~60.000μg·ml-1(r=0.999 6)、18.000~90.000μg·ml-1(r=0.999 3);平均加样回收率分别为96.61%(RSD=1.40%),97.66%(RSD=1.27%)。结论:该方法简便易行、准确、重复性好,可用于四季感冒胶囊的含量控制。
Objective: To establish a method for the determination of hesperidin and forsythin in the four seasons cold capsule. Methods: The chromatographic column was AgilentXDB C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-0.1% phosphoric acid with gradient elution at a flow rate of 1.0 ml · min-1. The column temperature was 30 ℃, detection wavelength of 277nm, injection volume of 5μl. Results: The linear ranges of hesperidin and forsythin were 12.000 ~ 60.000μg · ml-1 (r = 0.999 6), 18.000-90.000μg · ml-1 (r = 0.999 3) 96.61% (RSD = 1.40%), 97.66% (RSD = 1.27%). Conclusion: The method is simple, accurate, reproducible and can be used for the content control of the four seasons cold capsule.