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连续4 a调查了以袋繁和自繁方式生产的4个滇Ⅰ型不育系的11个群体,发现“可育株”的出现频率为0.087 1%,其表型与相应保持系一致。采用测交和分子标记技术分析了这些“可育株”的基因型,发现袋繁不育系群体存在核恢复基因位点基因型为rf/rf的“保持株”和Rf/rf的“回复株”2种类型,而自繁不育系群体还新出现基因型为RfRf的“纯合株”类型。与袋繁群体比,自繁群体出现“可育株”的频率提高了74.7%,其中“保持株”频率提高了42.6%,“回复株”频率提高了149.3%。基于不育系繁殖方法不同,不育系中出现“可育株”混杂的几率不同,为获得高纯度的不育系种子,滇I型不育系繁殖应在袋繁基础上,扩大自繁群体,减少自繁次数。
Four populations of four Dian I-type CMS lines, which were produced in bag-blown and self-propagated ways, were investigated for 4 consecutive days. The frequency of occurrence of “fertile plants” was 0.087 1%, and its phenotype and corresponding maintainer Consistent. The genotypes of these “fertile plants” were analyzed by using the techniques of cross and molecular markers. The results showed that there were “maintainer lines” with rf / rf genotypes and nuclear Rf / rf “strains ” 2 types, and self-breeding sterile line also genotype RfRf “homozygous ” type. The frequency of “fertile plants ” increased by 74.7% compared with that of the wild breeds, with the frequency of “keeping the plant” increased by 42.6% and the frequency of “the recovery of plant” increased by 149.3%. Based on different breeding methods of sterile lines, there is a different probability of “fertile plants” mixed in sterile lines. In order to obtain high purity sterile seeds, the breeding of Yunnan type I sterile lines should be expanded on the basis of bag breeding Since the fan population, reduce the frequency of self-propagation.