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用改良Lakeman免疫斑点法测定85例散发性脑炎病人脑脊液中单纯疱疹病毒抗原，从中诊断单纯疱疹脑炎（HSE）20例（23．5％），表明能较早期诊断HSE。并对本法的几个关键环节作了理论阐述。
Herpes simplex virus (CSF) antigen in cerebrospinal fluid of 85 patients with sporadic encephalitis was detected by modified Lakeman immunofluorescence assay. 20 cases (23.5%) of herpes simplexemorrhagic encephalitis (HSE) were diagnosed, indicating that HSE could be diagnosed earlier. And made a theoretical exposition of several key links of this law.