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目的 :观察凝血酶对大鼠星形胶质细胞组织因子活性表达的影响及其机制。方法 :培养Wistar乳鼠星形胶质细胞 ,细胞用抗神经胶质纤维酸性蛋白单克隆抗体确认。细胞冻融液组织因子活性检测采用一期血浆复钙时间法。结果 :与对照组比较 ,凝血酶使星形胶质细胞组织因子表达明显增高 (P <0 .0 5 )。当凝血酶与三氟吡啦嗪或H7联用时 ,它对星形胶质细胞表达组织因子活性的刺激作用受到抑制。与凝血酶组比较 ,已酮可可碱十凝血酶组星形胶质细胞组织因子活性表达明显下降。结论 :凝血酶能刺激星形胶质细胞表达组织因子活性 ,三氟吡啦嗪、H7、己酮可可碱可抑制凝血酶的这种刺激作用。
Objective: To investigate the effect of thrombin on the expression of tissue factor in rat astrocyte and its mechanism. Methods: Wistar astrocytes were cultured and the cells were confirmed by anti-glial fibrillary acidic protein monoclonal antibody. Cell freeze-thaw fluid tissue factor activity test using a period of plasma calcium complex time method. Results: Compared with the control group, thrombin significantly increased the expression of tissue factor in astrocytes (P <0.05). When thrombin is used in combination with trifluoperazine or H7, its stimulation of astrocyte-expressed tissue factor activity is inhibited. Compared with the thrombin group, pentoxifylline decahalukase group astrocyte tissue factor activity was significantly decreased. CONCLUSION: Thrombin can stimulate the activity of tissue factor in astrocyte. Thiophenol, H7 and pentoxifylline can inhibit this stimulating effect of thrombin.