论文部分内容阅读
目的探讨全自动高通量与半自动ELISA检测HBV血清标志物临床应用效果。方法检测86例HBV感染者(均来源于门诊及住院的临床患者)﹑50例正常人血清(均来源于本院健康体检人群),采用全自动高通量和半自动ELISA检测HBV血清标志物。结果全自动法CV值(HBs Ag 3.67%、HBs Ab 5.55%、HBe Ag 3.52%、HBe Ab 8.28%、HBc Ab 18.00%)均小于半自动法(HBs Ag 7.10%、HBs Ab 11.12%、HBe Ag 7.98%、HBe Ab 11.83%、HBc Ab 20.23%)(P<0.05)。全自动法符合率为97.01%大于半自动法的85.23%(P<0.05)。结论半自动和全自动相比不利于提升HBV血清标志物ELISA检测的质量和标准化,同时缺少平稳度、重复性和准确度。
Objective To investigate the clinical application of automatic high-throughput and semi-automatic ELISA in the detection of HBV serum markers. Methods Totally 86 HBV infected patients (all outpatients and inpatients) and 50 healthy controls (all from our hospital) were enrolled in this study. Serum HBV markers were detected by automatic high-throughput and semi-automatic ELISA. Results The CV values (HBs Ag 3.67%, HBs Ab 5.55%, HBe Ag 3.52%, HBe Ab 8.28%, HBc Ab 18.00%) were all less than those of semi-automatic method (HBs Ag 7.10%, HBs Ab 11.12%, HBe Ag 7.98 %, HBe Ab 11.83%, HBc Ab 20.23%) (P <0.05). The coincidence rate of automatic method was 97.01%, which was greater than that of semi-automatic method (85.23%, P <0.05). Conclusions Semi-automatic and fully automated methods are not conducive to improving the quality and standardization of ELISA for HBV serum markers, while lacking in stability, repeatability and accuracy.