为探讨用腺病毒载体携带ＰＭＬ（ＰｒｏｍｙｅｌｏｃｙｔｉｃＬｅｕｋｅｍｉａ）基因作为前列腺癌基因治疗的可能性，应用重组人携带ＰＭＬ基因腺病毒（ＡｄＰＭＬ）感染培养的前列腺癌细胞，观察表达ＰＭＬ蛋白的癌细胞与对照组癌细胞的体外生长和裸鼠体内致瘤能力变化，对荷瘤裸鼠瘤体周围注射ＡｄＰＭＬ，观察治疗组和对照组肿瘤生长的变化。结果显示，感染ＡｄＰＭＬ的前列腺癌细胞体外生长和裸鼠体内致瘤能力明显下降，荷瘤裸鼠瘤体周围注射ＡｄＰＭＬ后肿瘤生长速度明显减慢。证实了ＰＭＬ是一种生长抑制因子，提示其可能被应用于前列腺癌的基因治疗研究 In order to explore the possibility of adenovirus vector carrying PromyelocyticLeukemia (PML) gene as a gene therapy for prostate cancer, recombinant human PML adenovirus (AdPML) was used to infect cultured prostate cancer cells and the expression of PML protein in cancer cells was compared with the control The growth of cancer cells in vitro and tumorigenic ability of nude mice in vivo were studied. Ad-PML was injected into tumor-bearing nude mice, and the changes of tumor growth in treatment group and control group were observed. The results showed that in vitro growth of Ad-PM-infected prostate cancer cells and nude mice in vivo tumorigenic ability decreased significantly, tumor-bearing nude mice around the injection of Ad  PMML after tumor growth was significantly slower. Confirmed that PML is a growth inhibitory factor, suggesting that it may be applied to the study of gene therapy of prostate cancer