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研究目的在于为以后控制重要农艺性状的QTL定位、梨分子标记辅助育种及品种改良提供基础理论。利用‘新世纪梨’ב崇化大梨’杂交得到的210株F1代实生苗为作图群体,对分离群体进行了ISSR、SRAP、SSR标记的多态性检测,共得到154条多态性条带,其中偏离孟德尔遗传比例的含21.4%(P<0.01)。应用JoinMap 4.0软件对154个多态性条带进行遗传连锁分析,构建了一张包括9个SSR标记,79个SRAP标记,8个ISSR标记,合计96个标记分属于14个连锁群的遗传图谱,图谱总长度为1530cM,平均图距为16.1 cM,最大的连锁群含有64个标记,最小遗传距离小于0.1cM。
The purpose of this study is to provide the basic theory for the future control of QTL mapping of important agronomic traits, pear molecular marker-assisted breeding and breed improvement. A total of 210 F1 generation seedlings were obtained from the cross of ’New Century pear’ × ’Chong’daidaoli’. The ISSR, SRAP and SSR markers were used to detect the segregation population. A total of 154 polymorphisms Sexual bands, with a deviation from Mendelian genetic ratio of 21.4% (P <0.01). The genetic linkage analysis of 154 polymorphic bands was carried out by using JoinMap 4.0 software to construct a genetic map including 9 SSR markers, 79 SRAP markers and 8 ISSR markers, and a total of 96 markers belonging to 14 linkage groups , The total length of the map is 1530cM, the average distance is 16.1 cM, the largest linkage group contains 64 markers, the minimum genetic distance is less than 0.1cM.