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目的探索ｎｍ２３＿Ｈ１ｍＲＮＡ在胃癌中的表达程度与转移及预后的关系．方法应用原位分子杂交（ＩＳＨ）技术，检测胃癌组织１１２例（男８４例，女２８例，年龄２９岁～７０岁）中ｎｍ２３＿Ｈ１ｍＲＮＡ的表达，并结合临床资料进行分析．结果胃癌肿瘤区、移行区及癌旁区ｎｍ２３＿Ｈ１ｍＲＮＡ阳性表达率分别为４２９％，２７６％和１８％，阳性表达呈梯度下降趋势；无淋巴结转移的胃癌ｎｍ２３＿Ｈ１ｍＲＮＡ表达阳性为８７２％（４１／４７），有淋巴结转移的胃癌为１０８％（７／６５）；随访８８例中，≥３年生存者中６８６％（２４／３５）ｎｍ２３＿Ｈ１ｍＲＮＡ表达阳性，＜３年生存者中３７９％（１１／２９）ｎｍ２３＿Ｈ１ｍＲＮＡ表达阳性，故ｎｍ２３＿Ｈ１ｍＲＮＡ阳性表达在胃癌患者的淋巴结转移、非转移中差异显著（Ｐ＜００１）．结论ｎｍ２３＿Ｈ１ｍＲＮＡ表达率的升高与淋巴结的转移呈负相关性，与胃癌患者生存期呈正相关性
Objective To explore the relationship between the expression of nm23_H1 mRNA and its metastasis and prognosis in gastric cancer. Methods In situ hybridization (ISH) technique was used to detect the expression of nm23_H1 mRNA in 112 patients with gastric cancer (84 males and 28 females, aged 29 to 70 years). Results The positive expression rate of nm23_H1 mRNA in tumor area, transitional area and paracancerous area of gastric cancer were 42.9%, 27.6% and 18%, respectively. The positive expression of nm23_H1 mRNA showed a gradient decreasing trend. The positive expression rate of nm23_H1 mRNA in gastric cancer without lymph node metastasis was 87. 2%. (41/47), gastric cancer with lymph node metastasis was 10.8% (7/65); in 88 patients with follow-up, 68.6% (24/35) of nm23_H1 mRNA was positive in ≥ 3 year survival, and <3 years survival. Among them, 37.9% (11/29) of nm23_H1 mRNA expression was positive, so the positive expression of nm23_H1 mRNA was significantly different in lymph node metastasis and non-metastasis of gastric cancer patients (P<001). Conclusion The increased expression of nm23_H1 mRNA is negatively correlated with lymph node metastasis and positively correlated with the survival time of gastric cancer patients.