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目的对茅苍术基因组大小及染色体特征开展研究,以期为阐明茅苍术遗传规律及促进茅苍术良种选育工作提供基础。方法以茅苍术种子和幼叶为材料,利用流式细胞术及核型分析技术开展研究。结果茅苍术染色体数目2n=24,核型公式2n=2x=24=6m+18sm(2SAT),基因组2C值为9.73pg,基因组值为9.52×109bp。结论该研究为茅苍术基因组大小和染色体研究提供了数据参考,对茅苍术基因组研究和新品种选育有重要意义。
Objective To study the genomic size and chromosome characteristics of Atractylodes macrocephala so as to provide a basis for elucidating the genetic rules of Atractylodes macrocephala and promoting the breeding of Atractylodes. Methods Atractylodes lancea seeds and young leaves were used as materials, and flow cytometry and karyotyping techniques were used to study them. Results The chromosome number of Atractylodes lancea was 2n = 24 and the karyotype formula was 2n = 2x = 24 = 6m + 18sm (2SAT). The genomic 2C value was 9.73pg and the genomic value was 9.52 × 109bp. Conclusion This study provides data reference for the genome size and chromosome of Atractylodes lancea, and is of great significance to genomic research and breeding of new varieties of Atractylodes.