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采用实验室模拟培养、测定剩余尿素量的方法,对醋酸棉酚作为一种新型脲酶抑制剂进行初步研究,并探讨醋酸棉酚对红壤脲酶活性抑制作用的影响因素。结果表明,醋酸棉酚与尿素混合施用,培养2d,1%醋酸棉酚(相对于尿素质量)的脲酶抑制率为53.57%。土壤持水量对脲酶活性有较大影响,且在脲酶活性高时,醋酸棉酚的脲酶抑制作用更强。随着温度的升高,醋酸棉酚对土壤脲酶的抑制作用越来越明显,且表现出混合性抑制的特征。醋酸棉酚提前施用7d,尿素回收率从混合施用的73.64%增加到99.00%,说明改进施用方式可大幅度提高醋酸棉酚的脲酶抑制效果。
A laboratory simulation culture method was used to determine the amount of urea remaining. A preliminary study on gossypol acetate as a novel urease inhibitor was carried out. The factors affecting the inhibition of urease activity in red soil were also discussed. The results showed that the urease inhibition rate was 53.57% when mixed with urea and gossypol acetate for 2 days, 1% gossypol acetate (relative to urea). Soil water content had a significant effect on urease activity, and urease inhibition was stronger when urease activity was high. With the increase of temperature, the inhibitory effect of gossypol acetate on soil urease was more and more obvious, and showed the characteristics of mixed inhibition. Acetate gossypol was pre-applied for 7 days. The recovery rate of urea increased from 73.64% to 99.00% of the mixed application, indicating that improving the application mode can greatly increase the urease inhibition effect of gossypol acetate.