目的了解浙江地区无偿献血人群HCV基因型分布情况。方法对2011年11月~2012年12月的献血者血样进行ELISA和核酸检测双重筛选,得到双阳性高滴度标本24例。通过逆转录反应得到cDNA后,用巢式PCR(nestedPCR),扩增分型最佳区域的NS5B基因片段(于RNA指导的聚合酶相关)以及基因组内变异率最高区域之一的E1基因片段(于包膜糖蛋白相关)。扩增所得产物进行核苷酸序 Objective To understand the distribution of HCV genotypes in unpaid blood donors in Zhejiang Province. Methods Blood samples of blood donors from November 2011 to December 2012 were screened by ELISA and nucleic acid double screening, and 24 double-positive high-titer samples were obtained. After cDNA was obtained by reverse transcription reaction, the nested PCR (nested PCR) was used to amplify the NS5B gene fragment (RNA-directed polymerase-related) in the best-genotyped region and the E1 gene fragment (one of the most highly mutated regions in the genome Related to envelope glycoproteins). The resulting product is amplified for nucleotide sequencing