论文部分内容阅读
目的利用骨组织计量学方法，从骨吸收与骨形成活动的组织计量学参数的变化情况，了解实验动物血清和骨微环境中ＩＬ－６与绝经后骨质疏松的关系。方法４０只雌性４月龄ＳＤ大鼠，随机分为实验组和对照组。分别于术后４、１０、１６、２２周处死一组大鼠。用ＥＬＩＳＡ方法测得血清和骨组织培养液中ＩＬ－６的浓度。利用计算机图像分析系统考察不脱钙骨组织切片的ＴＢＶ、ＭＴＴ、ＦＳ、ＲＳ、ＬＳ、和ＭＡＲ。结果相关关系分析提示：血中ＩＬ－６仅与ＲＳ呈正相关。而骨组织培养液中ＩＬ－６变化与ＲＳ、ＦＳ呈正相关。与ＭＴＴ和ＴＢＶ呈负相关。依据相关系数的大小，我们发现：骨组织中ＩＬ－６浓度与骨形态计量学参数的相关程度ＲＳ＞ＭＴＴ＞ＦＳ＞ＴＢＶ。结论本研究采用骨组织计量学的方法，在组织学水平上证实了绝经后血清和骨组织中ＩＬ－６的水平增高，使破骨细胞的骨吸收活动增强，导致了骨质疏松的发生。同时也为利用分子生物学技术，如使用ＩＬ－６及ＩＬ－６受体拮抗剂等方法治疗绝经后骨质疏松症提供了研究的方法
OBJECTIVE: To investigate the relationship between IL-6 and postmenopausal osteoporosis in serum and bone microenvironment of experimental animals based on histomorphometric parameters of bone resorption and bone formation using bone histomorphometry. Methods 40 female 4-month-old SD rats were randomly divided into experimental group and control group. A group of rats were sacrificed at 4,10,16,22 weeks after operation. The concentration of IL-6 in serum and bone tissue culture fluid was measured by ELISA. The TBV, MTT, FS, RS, LS, and MAR of non-decalcified bone tissue sections were examined by computer image analysis system. Results Correlation analysis suggested that IL-6 in blood was only positively correlated with RS. The change of IL-6 in bone tissue culture fluid was positively correlated with RS and FS. Negatively correlated with MTT and TBV. Based on the correlation coefficient, we found that the correlation between IL-6 concentration in bone tissue and bone morphometry was RS> MTT> FS> TBV. Conclusion In this study, bone histomorphometry was used to confirm the increase of IL-6 level in serum and bone tissue at postmenopausal stage, which enhanced the bone resorption activity of osteoclasts and led to the occurrence of osteoporosis. At the same time, it also provides a research method for the treatment of postmenopausal osteoporosis by using molecular biology techniques such as IL-6 and IL-6 receptor antagonists