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目的比较肿瘤转移相关基因(MTA1)在人骨肉瘤细胞高低转移株的表达水平,探讨MTA1表达水平与骨肉瘤细胞转移潜能的相关性。方法采用半定量逆转录-聚合酶链反应 (RT-PCR)检测MG-63骨肉瘤细胞高低转移株MTA1的表达情况,用Boyden小室体外侵袭实验检测两株MG-63细胞的体外侵袭力;用脂质体介导的MTA1基因转染MG-63低转移株细胞,通过 RT-PCR检测MTA1的表达;Boyden小室体外侵袭实验检测转染前后细胞侵袭力的变化。结果 RT-PCR结果显示MTA1在MG-63低转移细胞株中表达水平低(1．32),在高转移细胞株中表达水平高(6．27,P<0．05);Boyden小室体外侵袭实验显示MG-63高转移株细胞体外侵袭力强,其穿膜细胞相对百分率为(46．3±2．4)％,低转移株细胞体外侵袭力较弱,其穿膜细胞相对百分率(12．6± 1．1)％,两者差异有统计学意义(P<0．05);转染MTA1基因后,低转移细胞株转移潜能较未转染细胞明显增高。结论 MTA1与人骨肉瘤细胞转移潜能有密切关系。
Objective To investigate the expression of MTA1 in human osteosarcoma cell lines and to investigate the correlation between the expression level of MTA1 and the metastatic potential of osteosarcoma cells. Methods The expression of MTA1 in MG-63 osteosarcoma cells was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The in vitro invasion of two MG-63 cells was detected by Boyden chamber in vitro. Liposome-mediated MTA1 gene was transfected into MG-63 cells with low metastatic potential, and the expression of MTA1 was detected by RT-PCR. Boyden chamber invasion assay was used to detect the invasiveness of cells before and after transfection. Results The results of RT-PCR showed that the expression level of MTA1 was low (1.32) in MG-63 low metastatic cell lines and high in metastatic cell lines (6.27, P <0.05) The results showed that the invasiveness of MG-63 cells was highly invasive in vitro. The relative percentage of transmembrane cells in MG-63 cells was (46.3 ± 2.4)%. The invasiveness of MG-63 cells in vitro was weak. The relative percentage of transmembrane cells .6 ± 1.1)%, respectively. The difference between the two groups was statistically significant (P <0.05). After transfection of MTA1 gene, the metastatic potential of low metastatic cells was significantly higher than that of untransfected cells. Conclusion MTA1 is closely related to the metastatic potential of human osteosarcoma cells.