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笔者从１９９３年５月始在武汉市水果湖地区应用球形芽孢杆菌Ｃ（３－４１）地方株制剂，进行大面积控制致乏库蚊的现场研究。结果表明，用３ｍｌ／ｍ２球形芽孢杆菌Ｃ（３－４１）乳剂，对大型致乏库蚊孳生地每１０天处理１次，包括水塘、水沟、水槽及水坑；另用３ｇ／ｍ２块剂每月处理１次水缸和小容器等，可使幼虫密度下降３８～９９％，并能有效地控制成蚊密度和削平致乏库蚊成蚊的季节高峰。
From May 1993, the author applied the Bacillus subtilis C (3-41) local strain preparation to the fruit lake area in Wuhan City for field research on Culex pipiens pallenschuch as large area control. The results showed that with 3 ml / m2 B. sphaericus C (3-41) emulsion, the large-scale Culex pipiens pallens were treated once every 10 days, including reservoirs, ditches, sinks and puddles; the other 3 g / m2 The treatment of water tank and small container once a month by the blocker can reduce the density of larvae by 38-99% and effectively control the density of adult mosquitoes and the peak season of mosquito larvae.