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用激光镊子拉曼光谱法优化红法夫酵母生产虾青素的条件。首先,将红法夫酵母细胞拉曼光谱与虾青素标准品溶液拉曼光谱进行对比,找出定量虾青素的拉曼特征峰;然后对照红法夫酵母生长曲线与不同时间点红法夫酵母细胞内虾青素含量曲线,进行发酵时间的优化;最后将红法夫酵母分别用不同氮源、碳源培养基培养,对比其细胞的虾青素拉曼特征峰强度,最终优化培养基。由实验可得,适宜定量虾青素的拉曼特征峰是1520cm-1峰;最佳发酵时间为72h;最佳优化培养基氮源含硫酸铵4g/L+硝酸钾4g/L,碳源含蔗糖60g/L。从上述结果可知,用激光镊子拉曼光谱法对红法夫酵母合成虾青素的条件进行优化,充分发挥了其操作简便、耗时短、样品用量少、对样品无损伤等优势,使所得结果更准确可信。因此,激光镊子拉曼光谱法是红法夫酵母细胞合成虾青素条件优化的理想选择。
Optimization of Astaxanthin Production by Astragalus Using Laser Tweezers Raman Spectroscopy. First, comparing the Raman spectra of Phaffia rhodozyma cells with the Raman spectrum of the astaxanthin standard solution to find out the Raman peak of astaxanthin quantitatively; then, comparing the growth curve of Phaffia rhodozyma with the red method at different time points Yeast cells within the astaxanthin content curve, the fermentation time optimization; Finally, the Rhizophora yeast were cultured with different nitrogen sources, carbon source medium, compared to the cells of the astaxanthin Raman peak intensity, the final optimization of culture base. According to the experiment, the Raman peak of the suitable quantitative astaxanthin is 1520cm-1; the optimum fermentation time is 72h; the optimum nitrogen source of the optimized medium is ammonium sulfate 4g / L + potassium nitrate 4g / L, carbon source Sucrose 60g / L. From the above results, the laser tweezers Raman spectroscopy synthesis of Astragalus yeast astaxanthin optimized conditions, give full play to its simple, time-consuming, less sample, the sample without damage and other advantages, so that The result is more accurate and credible. Therefore, laser tweezers Raman spectroscopy is an ideal choice for the optimization of astaxanthin production conditions in Phaffia rhodozyma cells.