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对膜荚黄芪(Astragalus membranaceus)根甲醇提取物中的抗氧化活性成分进行了分离和鉴定。首先将膜荚黄芪根用甲醇提取,而后再用乙醚提取,乙醚组分经硅胶柱层析分离(以苯-乙酸乙酯作为洗脱液)。所得4种成分用亚麻酸空气氧化法检测其抗氧化活性,所采用的指标为空气氧化(恒温60℃,通空气500ml/min,共2h)后亚麻酸的过氧化物值(POV)、硫代巴比妥酸值(TBAV)。结果
The antioxidative active components in root methanol extract of Astragalus membranaceus were isolated and identified. First, the root of Scutellaria baicalensis Georgi was extracted with methanol and then extracted with ether. The ether component was separated by silica gel column chromatography (benzene-ethyl acetate as eluent). The four components were tested for their antioxidant activity by linolenic acid air oxidation. The indicators used were the peroxide value (POV) of linolenic acid and sulfur after air oxidation (constant temperature 60°C, ventilation air 500ml/min, total 2h). Debarbituric acid (TBAV). result