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用子叶接种法鉴定了来自黑龙江、内蒙古、湖北、以及四川的65个大豆品种对疫霉根腐病1号生理小种的抗感情况。其中抗病品种11个,中间类型5个,感病品种49个。其鉴定结果与下胚轴接种法比较相同的有51个品种。经过DPS v7.05分析下胚轴接种法与子叶接种法之间的相关呈极显著,经r×c独立性测验得到χ2=1.46<χ0.05,22=5.99,2种方法差异不显著。证明了子叶接种法进行大豆疫霉根腐病抗性鉴定同样准确可行。此外还可以解决遗传分析上需要保存感病植株后代的问题,为遗传分析时鉴定疫霉根腐病抗感情况提供了可靠的方法。
The resistance of 65 soybean cultivars from Heilongjiang, Inner Mongolia, Hubei and Sichuan to Physiological race 1 of Phytophthora root rot was identified by inoculation of cotyledons. Among them, 11 resistant varieties, 5 intermediate types and 49 susceptible varieties. The identification results and hypocotyls inoculation method has the same 51 varieties. After DPS v7.05 analysis, the correlation between hypocotyls inoculation method and cotyledon inoculation method was extremely significant. Χ2 = 1.46 <χ0.05, 22 = 5.99 by r × c independence test. There was no significant difference between the two methods. Proved cotyledon inoculation method for soybean root rot resistance identification is also accurate and feasible. In addition, the problem of preserving the offspring of susceptible plants in genetic analysis can also be solved, providing a reliable method for identifying anti-flu susceptibility of Phytophthora capsici during genetic analysis.