论文部分内容阅读
探讨不同pH模拟酸雨对葱兰主要生理特性的影响及Ca2+的调控作用。通过盆栽试验,研究10mmol/LCa(NO3)2处理后不同pH(2.0、2.5、3.0、4.0和5.6)模拟酸雨胁迫对葱兰的有机物代谢、膜系统稳定性、抗氧化酶活性、叶绿素(Chl)及气体交换参数的影响。结果表明:随着pH的降低,葱兰叶片相对电导率(γ)、丙二醛(MDA)和超氧化物歧化酶(SOD)逐渐升高,可溶性蛋白、可溶性糖含量和过氧化物酶(POD)逐渐下降;Chl和净光合速率(Pn)随pH的降低而降低,气孔导度(Gs)和胞间CO2浓度(ci)先随pH的降低而升高,当pH 2.0时显著降低;在pH≤4.0时,可溶性糖与pH呈显著正相关,SOD和Pn均与pH呈显著负相关。同一强度酸雨胁迫下,经10mmol/L Ca(NO3)2处理后,葱兰叶片中可溶性蛋白、可溶性糖、POD、SOD、Chl和Pn均有不同程度的升高,γ和MDA显著降低,且各指标的变化随pH的降低变化不明显。模拟酸雨胁迫下,Ca2+能增强葱兰各项生理功能的稳定性,从而减轻酸雨对葱兰的伤害,试验还发现,Ca2+对葱兰的调控作用在强酸度(pH≤3.0)胁迫下,效果更明显。
Effects of different simulated acid rain on the main physiological characteristics of Cucumber and the regulation of Ca2 + were discussed. Pot experiments were conducted to study the effects of simulated acid rain stress on organic matter metabolism, membrane system stability, antioxidant enzyme activities, chlorophyll (Chl ) And gas exchange parameters. The results showed that the relative conductivity (γ), malondialdehyde (MDA) and superoxide dismutase (SOD) increased gradually with the decrease of pH. The contents of soluble protein, soluble sugar and peroxidase POD decreased gradually; Chl and net photosynthetic rate (Pn) decreased with the decrease of pH, while stomatal conductance (Gs) and intercellular CO2 concentration (ci) increased with the decrease of pH, At pH≤4.0, there was a significant positive correlation between soluble sugar and pH, while both SOD and Pn were negatively correlated with pH. Under the same acid rain stress, soluble protein, soluble sugar, POD, SOD, Chl and Pn increased in different degrees after treatment with 10 mmol / L Ca (NO3) 2, while γ and MDA decreased significantly The change of each index did not change with the decrease of pH. Simulated acid rain stress, Ca2 + can enhance the stability of various physiological functions of Sclareolum and thus reduce the acid rain on Sclerotium. The experiment also found that Ca2 + regulation of Sclerotium under strong acidity (pH≤3.0) stress, the effect More obvious.