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用丙肝病毒Ｃ＋Ｅ１区真核表达质粒ｐｃＤＮＡ－ＨＣＶ／Ｃ＋Ｅ１按４００ｕｇ／只剂量免疫ＢＡＬＢ／Ｃ小鼠，１４周后同一剂量再加强免疫一次。加强免疫后２周，在５０％ＰＥＧ１４５０介导下将脾细胞与ＳＰ２／０小鼠骨髓瘤细胞（５１）融合。实验结果融合率达５４．３％（３１３／５７６）。阳性率为５．４％（１７”３１３）。克隆化后得到６株稳定分泌抗丙肝病毒Ｃ区单克隆抗体杂交瘤细胞株。这６株杂交瘤均产生ＩｇＭ抗体，接种ＢＡＬＢ／Ｃ小鼠后产生腹水的效价为１６４－１３２０（ＥＬＩＳＡ）。
BALB / C mice were immunized with the eukaryotic expression plasmid pcDNA-HCV / C + E1 of C + E1 region of hepatitis C virus at a dosage of 400 ug / mouse. After 14 weeks, the same dose was boosted once more. Two weeks after booster immunization, splenocytes were fused with SP2 / 0 mouse myeloma cells (51) under 50% PEG1450-mediated. The experimental results of fusion rate of 54.3% (313/576). The positive rate was 5.4% (17 "313) .After cloning, 6 strains of monoclonal antibody secreting monoclonal antibodies against hepatitis C virus (C) were stably secreted.The BALB / C mice were inoculated with IgM antibody Post-ascites titer was 164-1320 (ELISA).