论文部分内容阅读
本研究选用无细胞核而富含珠蛋白mRNA的正常小鼠网织红细胞与骨髓瘤细胞系中的BW—胸腺淋巴肉瘤细胞系,NS—1浆细胞瘤细胞系和P388淋巴瘤细胞等分别进行胞质杂交的实验,以研究胞质因子调控肿瘤细胞恶性的可能性。实验结果表明三种淋巴瘤细胞在并入网织红细胞胞质体后所形成的杂交细胞呈现下列去恶性化的特征:1.杂交细胞出现组织化学反应及超微结构的形态改变;2.细胞分裂指数及生长速度明显下降;3.作异种移植至裸鼠后丧失发生肿瘤的能力;4.染色体数量明显减少,畸变率下降;5.出现基因产物血红蛋白的联苯胺阳性反应,并在BW—R杂交细胞传20多代过程中持续出现。对胞质体杂交细胞中基因产物血红蛋白的连续表现,染色体的丢失和在异种接种中丧失长瘤能力的可能机理予以讨论。
In this study, normal mouse reticulocyte rich in globin mRNA and BW-thymic lymphoma, NS-1 plasmacytoma cell line and P388 lymphoma cells in myeloma cell line were selected for cell culture In order to study the possibility of cytoplasmic factors regulating malignant tumor cells. The experimental results show that the hybridoma cells formed by the three kinds of lymphoma cells after reticulocyte assembly into cytoplasm have the following features of demalignization: 1. The histochemical reaction of hybrid cells and the ultrastructural changes; 2. Cell division Index and growth rate decreased significantly; 3 for xenograft to nude mice loss of tumor-generating capacity; 4. Chromosome number was significantly reduced, the rate of distortion decreased; 5. The gene product hemoglobin benzidine positive reaction, and BW-R Hybrid cells pass on for more than 20 generations. The continuous presentation of the gene product hemoglobin in hybridoma cells, the loss of chromosomes, and the possible mechanism for loss of tumor length in xenograft vaccines are discussed.