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目的:探讨益气消癥法方药含药血清干预EA.hy926细胞增殖,抑制子宫肌瘤血管新生的作用机理。方法:观察益气消癥法方药含药血清对体外培养的EA.hy926细胞增殖、迁移、凋亡的影响。实验分为5组:空白血清组;RU486组;中药高剂量组;中药中剂量组;中药低剂量组。采用MTT法检测EA.hy926细胞的增殖情况,细胞划痕法检测细胞迁移率,流式细胞术法检测细胞的凋亡率。结果:益气消癥法方药可抑制EA.hy926细胞增殖、迁移、凋亡。结论:益气消癥法方药可抑制EA.hy926的细胞的增殖,诱导其迁移、凋亡,进而控制肌瘤血管新生,减少肌瘤血液供应,最终达到缩消子宫肌瘤、改善临床症状的作用。
Objective: To investigate the mechanism of Yiqi Xiaoyan Prescription drug-containing serum on the proliferation of EA.hy926 cells and inhibition of angiogenesis in uterine fibroids. Methods: The effects of Yiqi Xiezheng Prescription Drug-containing serum on the proliferation, migration and apoptosis of cultured EA.hy926 cells were observed. The experiment was divided into 5 groups: blank serum group; RU486 group; Chinese medicine high dose group; Chinese medicine middle dose group; Chinese medicine low dose group. The proliferation of EA.hy926 cells was detected by MTT assay. The cell migration rate was detected by cell scratch assay and the apoptosis rate was detected by flow cytometry. Results: Yiqi Xiaozheng Prescription can inhibit the proliferation, migration and apoptosis of EA.hy926 cells. Conclusion: Yiqi Xiaozhengfang Prescription can inhibit the proliferation of EA.hy926 cells, induce its migration and apoptosis, and then control the angiogenesis of fibroids, reduce the fibroids blood supply, eventually achieve the cure of uterine fibroids and improve the clinical symptoms effect.