多形微眼藻在不同氮源条件下固碳途径的转录组解析及与其他微藻的比对

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本研究通过转录组技术对不同氮源培养下的多形微眼藻(Minutocellus polymorphus)固碳途径进行分析。通过对转录本进行NR、NT、Swiss-Prot和KOG富集分类及GO、KEGG相关代谢途径和基因差异表达分析,构建了多形微眼藻固碳途径。研究发现该藻除具有C3固碳途径外,还具有较完整的C4固碳通路。在注释到的164个固碳相关编码基因中,有33个出现明显表达差异。根据基因的差异表达情况可以发现,在有机氮源中多形微眼藻C3固碳途径的核酮糖-1,5-二磷酸再生阶段和C4固碳途径的磷酸烯醇式丙酮
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