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目的 :研究加味肾气丸对哮喘Th1、Th2细胞凋亡及表达细胞因子的影响。方法 :分离哮喘患者外周血CD4+细胞 ,分别以CCR5及CCR3和Th1 /Th2标志 ,检测其凋亡及INF -γ及IL -4表达 ,并观察加味肾气丸对其影响。结果 :空白对照与加味肾气丸 (浓度分别为 0 0 1、0 0 2、0 0 4g/ml)刺激体外培养支气管哮喘外周血CD4+细胞 2 4h分泌IL -4水平分别为 894 5 6± 3 1 0 2 2、480 0 5± 1 1 2 2 3、440 2 1± 1 0 5 1 1及 41 1 84± 1 0 3 2 5 pg/ml(与空白对照相比 ,p<0 0 5 ) ;Th2细胞凋亡率分别为 2 4 3 3± 3 2 9、3 2 68± 5 94、3 6 74± 5 44、3 8 5 8± 6 41 %(与空白对照相比 ,P <0 0 5或 0 0 1 ) ;与空白对照相比 ,不同浓度加味肾气丸对体外培养支气管哮喘患者CD4+细胞分泌INF -γ及Th1细胞凋亡无明显影响 (P <0 0 5 )。结论 :加味肾气丸通过诱导Th2细胞凋亡纠正哮喘Th1 /Th2失衡 ,抑制哮喘气道炎症
Objective : To study the effect of Jiawei Shenqi Pill on the apoptosis of Th1/Th2 cells and the expression of cytokines in asthma. METHODS: CD4+ cells in peripheral blood of asthmatic patients were isolated, and CCR5, CCR3 and Th1/Th2 markers were used to detect apoptosis and expression of INF-γ and IL-4. The effects of Jiawei Shenqi Pills were observed. RESULTS: The level of IL-4 secreted by peripheral blood CD4+ cells stimulated by stimulating bronchial asthma in vitro and Jiawei Shenqi Pill (concentrations 0 01, 0 0 2, 0 4 4 g/ml) was 89 4 56 6 ± 3 respectively. 1 0 2 2,480 0 5± 1 1 2 2 3,440 2 1± 1 0 5 1 1 and 41 1 84± 1 0 3 2 5 pg/ml (p<0 05 compared with blank control) The apoptotic rate of Th2 cells was 2 4 3 3 ± 3 2 9, 3 2 68 ± 5 94, 3 6 74 ± 5 44, 3 8 5 8 ± 6 41 % (P <0 0 compared with the blank control; 5 or 0 0 1 ) ; Compared with the control group, Jiawei Shenqi Pill at different concentrations had no effect on the secretion of INF - γ and Th1 cell apoptosis in CD4 + cells of patients with bronchial asthma in vitro (P <0 05). CONCLUSION : Jiawei Shenqi Pills corrects asthma Th1/Th2 imbalance by inducing apoptosis of Th2 cells and inhibits asthmatic airway inflammation