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目的 探讨99Tcm C5 0放免显像药盒的制备方法。方法 用 2 亚氨噻酚盐酸盐修饰C5 0 ,通过99Tcm 葡庚糖酸钠 (GH)转换 ,将99Tcm 标记在C5 0上。用HPLC法分析修饰抗体和标记抗体 ,用纸层析法测定标记率和胶体 ,并用免疫分析法测定标记抗体生物活性。裸鼠尾静脉注射抗体后进行显像。结果 C5 0标记率为 97% ,其中99Tcm 胶体含量为 4% ,修饰抗体 4℃下可保存 3个月 ,标记抗体有良好的稳定性和免疫活性。荷瘤裸鼠 2 4h肿瘤部位与四肢肌肉的放射性比值为 4.0 3。结论该制备方法高效、便捷 ,并可用于其他单抗放免显像药盒的制备。
Objective To investigate the preparation method of 99Tcm C5 0 radioimmunoimaging kit. Method C5 0 was modified with 2-iminothiophenol hydrochloride, 99Tcm labeled with C5 0 by 99Tcm sodium glucoheptonate (GH) conversion. The modified antibodies and labeled antibodies were analyzed by HPLC, the labeling rates and colloids were determined by paper chromatography, and the bioactivity of labeled antibodies was determined by immunoassay. Nude mice tail vein injection of antibodies after imaging. Results The C5 0 labeling rate was 97%. The 99Tcm colloid content was 4%. The modified antibody could be stored at 4 ℃ for 3 months. The labeled antibody had good stability and immunocompetence. The tumor-bearing nude mice had a radiation ratio of 4.03 at the site of the tumor to the limbs at 24 hours. Conclusion The preparation method is efficient and convenient, and can be used for the preparation of other monoclonal antibody immuno-imaging kits.