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目的 建立适用于研究抗青光眼药物降眼压作用及保护视神经作用的兔青光眼模型。方法 将 2 1只兔随机分为Ⅰ、Ⅱ、Ⅲ及Ⅳ组 ,分别对其前房内注射复方卡波姆、甲基纤维素及复方甲基纤维素 ,结膜下注射地塞米松诱发青光眼 ,并对 4种药物诱发的青光眼模型进行观察。结果 Ⅰ组高眼压持续 2 0～ 5 0d ,平均眼压为 2 9～ 35mmHg(1mmHg=0 133kPa) ,眼压峰值为 37～ 4 5mmHg,青光眼模型成功率为 91 7%。Ⅱ及Ⅲ组模型有 10 %～ 2 0 %的兔眼眼压升高持续 3～ 4d ,如按眼压为2 2mmHg持续 1周的标准判断 ,Ⅱ及Ⅲ组模型均不理想。Ⅳ组眼压平均升高 3mmHg ,持续 7d ,亦为失败模型。结论 复方卡波姆诱发的兔青光眼模型具有引起眼压中度、稳定升高的时间长 ,方法简单 ,易于操作和控制等优点。可用于对青光眼性视神经视网膜损害的研究及对抗青光眼药物的研究 ,是一种较理想的兔青光眼模型
OBJECTIVE To establish a rabbit glaucoma model that is suitable for the study of lowering the intraocular pressure (IOP) and protecting the optic nerve of anti-glaucoma drugs. Methods Twenty-one rabbits were randomly divided into groups Ⅰ, Ⅱ, Ⅲ and Ⅳ. The rabbits were injected with compound carbomer, methylcellulose and compound methylcellulose in the anterior chamber respectively, dexamethasone subconjunctival injection of glaucoma, Four drug-induced glaucoma models were also observed. Results The intraocular pressure (IOP) in group Ⅰ continued for 20 ~ 50 days, the mean IOP was 29 ~ 35mmHg (1mmHg = 0 133kPa), the peak IOP was 37 ~ 4 5mmHg, and the success rate of glaucoma model was 91.7%. In group Ⅱ and Ⅲ, 10% ~ 20% of intraocular pressure in rabbits increased for 3 ~ 4 days. According to the standard of intraocular pressure of 2 2mmHg for 1 week, the models of Ⅱ and Ⅲ were not ideal. Ⅳ group intraocular pressure increased 3mmHg on average, lasted 7d, also a failure model. Conclusions The rabbit model of glaucoma induced by compound carbomer has the advantages of moderate intraocular pressure, long time of stable increase, simple method, easy operation and control. It can be used to study glaucomatous optic nerve retina damage and research on anti-glaucoma drugs.It is an ideal rabbit model of glaucoma