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目的观察β-细辛醚对肠癌细胞株体外增殖抑制作用及其机制。方法①MTT法观察β-细辛醚对多种肿瘤细胞株的增殖的影响。②Transwell小室法,观察β-细辛醚对肠癌细胞株HT-29侵袭的影响;③粘附实验观察β-细辛醚对HT-29粘附能力的影响;④AnnexinV/PI双染法研究β-细辛醚对肠癌细胞株HT-29凋亡的诱导作用;⑤流式细胞仪检测β-细辛醚对肠癌细胞株HT-29细胞周期的影响;⑥JC-1荧光探针法检测线粒体膜电位水平的改变;⑦流式细胞仪检测β-细辛醚对肠癌细胞株HT-29活性氧水平的改变。结果①β-细辛醚对多种肿瘤细胞株有不同程度的抑制作用(P<0.05)。②β-细辛醚作用肠癌细胞HT-29后,细胞的侵袭和粘附能力明显降低(P<0.05)。③β-细辛醚作用肠癌细胞株HT-29后出现明显的早期凋亡及细胞周期明显改变。④β-细辛醚作用肠癌细胞株HT-29后线粒体膜电位降低及内活性氧水平升高。结论①β-细辛醚对肠癌细胞有明显的增殖抑制作用,能降低肠癌细胞HT-29的粘附和侵袭能力。②β-细辛醚可诱导肠癌细胞凋亡,改变细胞周期,其机制可能与线粒体凋亡途径有关。
Objective To observe the inhibitory effect of β-asarone on colon cancer cell lines in vitro and its mechanism. Methods 1MTT method was used to observe the effect of β-asarone on the proliferation of various tumor cell lines. 2 Transwell chamber method to observe the effect of β-asarone on the invasion of colon cancer cell line HT-29; 3 adhesion experiment to observe the effect of β-asarone on the adhesion ability of HT-29; 4AnnexinV/PI double staining study β -Induction of apoptosis of colon cancer cell line HT-29 by asarone;5Effect of β-asarone on cell cycle of colon cancer cell line HT-29 by flow cytometry;6JC-1 fluorescence probe assay Changes in mitochondrial membrane potential; 7 flow cytometry detection of β-asarone on colon cancer cell line HT-29 reactive oxygen levels. Results 1β-asarone had different degrees of inhibition on various tumor cell lines (P<0.05). After 2β-asarone acted on colon cancer cells HT-29, the invasion and adhesion of cells were significantly decreased (P<0.05). After 3β-asarone acted on colon cancer cell line HT-29, significant early apoptosis and obvious changes in cell cycle occurred. 4β-asarone reduced the mitochondrial membrane potential and increased the level of reactive oxygen species in colon cancer cell line HT-29. Conclusion 1β-asarone can significantly inhibit the proliferation of colon cancer cells, and can reduce the adhesion and invasion of colon cancer cells HT-29. 2β-asarone can induce apoptosis of colorectal cancer cells and change cell cycle, and its mechanism may be related to mitochondrial apoptosis pathway.