草菇多酚氧化酶（ｐｏｌｙｐｈｅｎｏｌｏｘｉｄａｓｅ）与过氧化物酶（ｐｅｒｏｘｉｄａｓｅ）的最适ｐＨ分别为６０～６８、６７～７４，最适温度分别为３０～３２℃、２５～２８℃在最适条件下，前者的活性远高于后者，当ｐＨ８６及温度４３℃或４８℃处理１ｍｉｎ，二者活力显著降低；但是，先在３５～３８℃保温１ｈ再测定，发现二者的最适温度分别达４０～４１℃、５２～５３℃，升温至８０℃１０ｓ，过氧化物酶仍表现活性Ｏ２对多酚氧化酶活性影响极大，而对过氧化物酶活性影响较小金属离子Ｍｎ２＋、Ｚｎ２＋、Ｍｇ２＋对二者均有强烈激活作用，Ｃａ２＋对二者的作用都很弱，Ｆｅ２＋、Ｃｕ２＋对前者作用明显强于后者二者的反应进程及酶浓度与反应速度的关系，表现出较大的差异 The optimal pH values ​​of polyphenoloxidase and peroxidase were 60 ~ 68, 67 ~ 74, respectively, and the optimum temperature were 30 ~ 32 ℃, 25 ~ 28 ℃  under the optimum conditions, the former activity is much higher than the latter, when pH8  6 and temperature 43 ℃ or 48 ℃ for 1min, both significantly reduced vitality; however, the first 35 ~ 38 ℃ incubated 1h and then The results showed that the optimum temperature of the two was 40 ~ 41 ℃, 52 ~ 53 ℃, respectively. When the temperature was raised to 80 ℃ for 10 seconds, the activity of peroxidase still had a great influence on the activity of polyphenol oxidase, The activity of enzyme was less affected. Mn2 +, Zn2 + and Mg2 + were strongly activated by both metal ions. The effect of Ca2 + on both was weak. The effect of Fe2 + and Cu2 + on the former was stronger than that of the latter And the relationship between enzyme concentration and reaction rate, showing a greater difference 