论文部分内容阅读
在本院苗圃(土壤为砂壤—轻壤土,未消毒,pH为8.6)用十种菌根菌的纯培养物,对油松(Pinus tabulaeformis)幼苗(出土后十天)进行人工接种试验,以便研究外生菌根对狞倒病的生物防治效应。其结果如下:1.通过接种试验,在供试的十个菌根真菌中,初步筛选出两个优良菌种:厚环粘盖牛肝菌[Suillus elegans(Fr.)Snell=S.grevillei]及S.sp.(带)。这些菌种对猝倒病不仅有生物防治作用,而且对苗木有较显著的促生长作用。2.所选出的菌种具有抗碱的能力,在碱性土壤上较好地发挥了抗病作用。3.在室内,采用琼脂平板测定法,测定菌根菌对病原立枯丝核菌(Rhizoct noia solani)的抑菌作用,试验结果证明:S.grevillei,S.sp.(带),Boletussp.(林)等菌根菌对立枯丝核菌具有较显著的消解,重寄生作用,从而抑制了立枯丝核菌的生长,室内的平板测定结果和菌根菌在田间所表现出来的生防效应基本上相吻合。
Artificial inoculation experiments were conducted on Pinus tabulaeformis seedlings (ten days after emergence) with pure culture of ten mycorrhizal fungi in our nursery (sandy soil-light loam soil, non-sterilized, pH 8.6) In order to study the biological control effect of ectomycorrhizal fungi on moth-feeding disease. The results are as follows: 1. Through the inoculation test, two excellent strains were initially screened out of the ten mycorrhizal fungi tested: Suillus elegans (Fr.) Snell = S.grevillei] And S.sp. (band). These strains not only biological control of damping off disease, but also on the seedlings have a more significant role in promoting growth. 2. The selected strains have the ability of alkali resistance, better play a role in alkaline soil resistance. In laboratory, Agar plate assay was used to determine the mycorrhizal bacteriostasis of Rhizoctonia solani. The results showed that S. gravillei, S.sp. (Band), Boletussp. (Lin) and other mycorrhizal fungi on Rhizoctonia solani more significant digestion, re-parasitization, which inhibits Rhizoctonia solani growth, indoor determination of the results of mycorrhizal fungi in the field and the field of biological protection The effect is basically consistent.