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目的:观察小檗碱对白介素-6(IL-6)诱导胰岛素抵抗的3T3-L1脂肪细胞脂联素表达的影响。方法:选用IL-6诱导3T3-L1脂肪细胞胰岛素抵抗(IR)模型。以20μg·L-1IL-6培养48 h,将3T3-L1脂肪细胞随机分为正常对照组、模型组、吡格列酮组(50μmol.L-1)和小檗碱高、中、低剂量组(10,20,50μmol.L-1),以葡萄糖氧化酶法测定葡萄糖消耗量,观察小檗碱对脂肪细胞葡萄糖摄取的影响,鉴定IR模型;采用实时荧光定量PCR技术测定脂肪细胞脂联素基因mRNA水平。结果:模型组葡萄糖消耗量及脂联素基因表达水平与正常对照组比较,均显著降低(P<0.05);小檗碱高、中、低剂量组及吡格列酮组均能显著增加葡萄糖消耗量及脂联素基因表达水平(P<0.05)。结论:小檗碱可增加白介素-6诱导胰岛素抵抗的3T3-L1脂肪细胞脂联素基因mRNA的表达,改善胰岛素抵抗状态。
Objective: To observe the effects of berberine on adiponectin expression in 3T3-L1 adipocytes induced by interleukin-6 (IL-6). Methods: 3T3-L1 adipocyte insulin resistance (IR) model was induced by IL-6. The 3T3-L1 adipocytes were randomly divided into normal control group, model group, pioglitazone group (50μmol.L-1) and berberine high, medium and low dose groups (10 , 20,50 μmol.L-1). The glucose consumption was measured by glucose oxidase method. The effect of berberine on glucose uptake was observed and the IR model was identified. Real-time quantitative PCR was used to determine adiponectin mRNA Level. Results: The levels of glucose consumption and adiponectin gene expression in model group were significantly lower than those in normal control group (P <0.05). Berberine high, medium and low dose groups and pioglitazone group could significantly increase glucose consumption and Adiponectin gene expression levels (P <0.05). Conclusion: Berberine can increase the mRNA expression of adiponectin in 3T3-L1 adipocytes induced by interleukin-6 and improve insulin resistance.