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考察大鼠肠道菌群对羊耳菊提取物中主要成分的代谢作用,将羊耳菊提取物与大鼠肠道菌液在厌氧条件下共同孵育24 h,通过正丁醇液液萃取处理后,采用色谱联用技术(UPLC-Q-TOF-MS/MS)对代谢产物进行定性分析,并结合布鲁克公司研发的数据处理工具Metabolite Tools,Data Analysis等对代谢信息进行综合分析。通过比较空白样品图谱,肠菌样品图谱以及两者的差异图谱,各色谱峰的准分子离子,碎片离子,分析羊耳菊提取物在大鼠肠道菌群作用下可能产生的代谢产物。结果提示,羊耳菊提取物在肠道菌群作用下,检测到14个代谢产物,主要包括单咖啡酰基奎宁酸的异构,水解的小分子化合物和咖啡酸的还原,甲基化和乙酰化等代谢产物,同时仅检测到1个二咖啡酰基奎宁酸的甲基化代谢产物。以此推测,咖啡酰基奎宁酸类成分在肠道菌群的作用下可能多数水解为分子量小,疏水性更强的代谢产物,使得其更容易被肠道吸收。
To investigate the metabolism of rat intestinal flora on the main constituents of the genus Pleuromum, the raceme of Pleurotus ferulae and rat intestinal bacteria were incubated under anaerobic conditions for 24 h, After processing, the metabolic products were qualitatively analyzed by chromatography-mass spectrometry (UPLC-Q-TOF-MS / MS) and Metabolite Tools, Data Analysis and other data processing tools developed by Bruker Company were used for comprehensive analysis of metabolic information. By comparing the blank sample map, enterotoxin sample map and the difference between the two maps, the peak of the quasi-molecular ions, fragment ions, the metabolites of the Pleurotus ostreatus extract in the rat intestinal flora may produce metabolites. The results showed that 14 strains of metabolites of Pleurotus ferulae extract were detected under intestinal microflora, including the isomerism of single caffeoylquinic acid, the hydrolysis of small molecule compounds and the reduction of caffeic acid, methylation and Acetylation and other metabolites, and only detected a dicaffeoylquinic acid methylation metabolites. It is speculated that caffeoylquinic acid components in the intestinal flora under the action of most likely to hydrolysis of small molecular weight, more hydrophobic metabolites, making it easier to be intestinal absorption.