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[Objective] To establish the isolation and determination methods of catechin from Cynomorium songaricum Rupr.[Method] The dried Cynomorium songaricum Rupr.powder was extracted with 50% ethanol through ultrasonic and purified with macroporous absorption resin.HPLC conditions were as follows:C18 column(250 mm×4.6 mm,5 μm);mobile phase of acetonitrile-0.2acetic acid(15:85 V/V);room temperature as column temperature;flow rate of 1ml/min,detective wavelength of 280 nm.[Result] Catechin was concentrated in 35% ethanol elution fraction;the sample and the negative control were separated well,the linear range of catechin was 0.005-0.04 mg/ml(r = 0.999 9),the recovery rate was 98%,RSD was 0.658%.[Conclusions] Catechin can be isolated and purified from Cynomorium songaricum Rupr.effectively;HPLC is suitable for the determination with the advantages of small deviation and good reproducibility.
[Objective] To establish the isolation and determination methods of catechin from Cynomorium songaricum Rupr. [Method] The dried Cynomorium songaricum Rupr.powder was extracted with 50% ethanol through ultrasonic and purified with macroporous absorption resin. HPLC conditions were as follows: C18 column (250 mm × 4.6 mm, 5 μm); mobile phase of acetonitrile-0.2 acetic acid (15:85 V / V); room temperature as column temperature; The sample and the negative control were separated well, the linear range of catechin was 0.005-0.04 mg / ml (r = 0.999 9), the recovery rate was 98%, the RSD was 0.658% . [Conclusions] Catechin can be isolated and purified from Cynomorium songaricum Rupr.effectively; HPLC is suitable for the determination with the advantages of small deviation and good reproducibility.