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目的从行为学及磁共振成像(MRI)表现上评价6-羟基多巴胺(6-OHDA)单侧毁损大鼠黑质致密部建立偏侧帕金森病(PD)模型的方法。方法70只SD大鼠随机分为3组:A组,30只,15μg/3μl黑质致密部(SNc)单点注射;B组,30只,30μg/6μlSNc单点注射;C组10只6μl含0.2%抗坏血酸的生理盐水SNc单点注射。术后不同时期观察阿扑吗啡(APO)腹腔注射诱导的大鼠旋转行为。6周后各组随机选取部分成模与非成模大鼠在高场强MRI下活体检测,观察大鼠颅内毁损情况。结果A、B组大鼠6-OHDA毁损黑质后,大部分成模大鼠在第3天可以检测到稳定的旋转(>210r/30min),其余成模大鼠在第14天可以检测到稳定的旋转。两组成模率相仿,成模率高达76.4%。第6周,MRI显示成模大鼠穿刺针道恰位于黑质部,而非成模大鼠穿刺针道偏离黑质部。结论应用6-OHDA单侧毁损大鼠黑质致密部可以高效、简便、稳定地制作PD大鼠模型;MRI检测可以客观评价其成模质量。
Objective To evaluate the establishment of a hemiparkinsonism (PD) model of substantia nigra in the unilateral lesion of 6-hydroxydopamine (6-OHDA) from behavioral and magnetic resonance imaging (MRI) Methods Seventy SD rats were randomly divided into 3 groups: group A, 30, single injection of 15μg / 3μl substantia nigra pars compacta (SNc); group B, 30, 30μg / 6μl SNc single injection; Saline containing 0.2% ascorbic acid SNc single injection. At different time points after operation, apomorphine (APO) intraperitoneal injection induced rat rotation behavior was observed. After 6 weeks, some of the rats in each group were selected randomly to in-mold and non-model rats for in vivo testing under high field MRI to observe the intracranial lesion in rats. Results After 6-OHDA damaged substantia nigra in group A and group B, most of the rats in model group could detect stable rotation (> 210r / 30min) on the third day, and the remaining model rats could detect on the 14th day Stable rotation. The two models have the same modulo rate and the modulo rate is as high as 76.4%. At the 6th week, MRI showed that the puncture needle of the model rats was located in the substantia nigra only, while the non-modeling rat puncture needle deviated from the substantia nigra. CONCLUSION: PD rat model can be made efficiently, easily and stably by using 6-OHDA unilateral lesion substantia nigra in rats. MRI can objectively evaluate the quality of the model.