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用细胞内记录方法检测２－氯－４－溴－α－甲基肉桂酸钠盐（ＳＣ１００１Ｎａ）和通道阻断剂四乙铵、氯化锰及河豚毒素对中华大蟾蜍离体缝匠肌动作电位和静息电位的影响。１０ｍｍｏｌ／Ｌ四乙铵及氯化锰对静息电位和动作电位幅度无影响，１μｍｏｌ／Ｌ河豚毒素完全阻断动作电位。２ｍｍｏｌ／ＬＳＣ１００１Ｎａ显著降低动作电位幅度并延长其持续时程（Ｐ＜０．０５），而对静息电位无影响，但其作用可逆。经四乙铵１０ｍｍｏｌ／Ｌ预处理后，２ｍｍｏｌ／ＬＳＣ１００１Ｎａ仍能延长动作电位持续时程，同时降低其幅度，而１０ｍｍｏ１／ＬＳＣ１００１Ｎａ完全阻断动作电泣，并使静息电位去极化（Ｐ＜０．０５）。且药物效应为不可逆。本研究结果提示：ＳＣ１００１Ｎａ可能是一种新型钠通道阻断剂。
The in vitro sartorius muscle action of Bufo bufo gargarizans was detected by intracellular recording method using 2-chloro-4-bromo-α-methylcinnamic acid sodium salt (SC1001Na) and channel blockers tetraethylammonium, manganese chloride and tetrodotoxin Potential and resting potential effects. 10mmol / L tetraethylammonium and manganese chloride had no effect on the resting potential and action potential amplitude, 1μmol / L tetrodotoxin completely blocked the action potential. 2mmol / LSC1001Na significantly reduced the amplitude of action potential and prolonged its duration (P <0.05), but had no effect on resting potentials, but its effect was reversible. After pretreatment with 10 mmol / L tetraethylammonium, 2 mmol / L LS100Na could prolong the duration of action potential and decrease its amplitude at the same time, while 10 mmo1 / LSC1001Na completely blocked the electrocuring and depolarization resting potentials (P < 0.05). And the drug effect is irreversible. The results suggest that: SC1001Na may be a new type of sodium channel blockers.