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目的观察Sirt3在原发性肝细胞癌(HCC)中的表达,并探讨过表达h Sirt3对人肝癌Hep G2细胞的存活及增殖影响。方法采用免疫组化法检测HCC组织和癌旁正常肝组织中Sirt3的表达;构建h Sirt3腺病毒载体,转染Hep G2细胞并过表达Sirt3蛋白,采用WST-1和Edu实验评价Sirt3对Hep G2细胞的存活及增殖作用。结果与正常肝组织比较,肝癌组织的Sirt3表达量明显降低;Ad-h Sirt3腺病毒感染Hep G2细胞后明显降低了细胞的存活率,抑制细胞生长。结论肝癌组织中Sirt3呈低表达或表达缺失,提示Sirt3的下调可能与HCC的发生密切相关;过表达Sirt3明显抑制Hep G2细胞株的存活及增殖,提示Sirt3可能是治疗肝癌的潜在靶点。
Objective To observe the expression of Sirt3 in primary hepatocellular carcinoma (HCC) and to investigate the effect of overexpression of h Sirt3 on the survival and proliferation of human hepatoma Hep G2 cells. Methods The expression of Sirt3 in HCC tissues and adjacent normal liver tissues was detected by immunohistochemistry. The hSirt3 adenovirus vector was constructed and transfected into Hep G2 cells and Sirt3 protein was overexpressed. The effects of Sirt3 on Hep G2 Cell survival and proliferation. Results Compared with normal liver tissue, the expression of Sirt3 in hepatocellular carcinoma was significantly decreased. Ad-h Sirt3 adenovirus infected Hep G2 cells significantly reduced cell viability and inhibited cell growth. Conclusions The low expression or absence of Sirt3 in HCC suggests that the down-regulation of Sirt3 may be closely related to the occurrence of HCC. Overexpression of Sirt3 significantly inhibits the survival and proliferation of Hep G2 cell lines, suggesting that Sirt3 might be a potential target for the treatment of HCC.