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目的研究子宫平滑肌瘤患者外周血淋巴细胞染色体变化情况。方法分别采用RP-MI1640＋20％小牛血清；Tc199＋20％小牛血清＋Fudr（最终浓度10（－7）mol／L）；TC199＋10％小牛血清＋Aphidicolin（0．2μmol／L）＋乙醇（最终浓度0．02％）培养液进行淋巴细胞培养，对每份样本至少观察100个淋巴细胞中期分裂相的染色体畸变及脆性位点表达的数量、类型及出现频率。结果子宫平滑肌瘤病人的淋巴细胞染色体畸变种类和畸变率明显高于对照组（P＜0．01）。在APC培养条件下，患者组淋巴细胞染色体的脆性位点表达率为70．2％，显著高于对照组的39．8％（P＜0．01）；并且患者组中有20种脆性位点在1／3以上个体中表达，对照组中仅有8种，而且分布特点亦不相同。结论子宫平滑肌瘤患者外周血淋巴细胞染色体不稳定性显著增加。
Objective To study the changes of peripheral blood lymphocytes in uterine leiomyoma patients. Methods Twenty-nine mice were treated with RP-MI1640 + 20% fetal bovine serum, Tc199 + 20% fetal bovine serum + Fudr (final concentration 10-7 mol / L), TC199 + 10% fetal bovine serum plus Aphidicolin (0.2 mol / L) 02%) culture medium for lymphocyte culture, observed at least 100 lymphocyte metaphase split chromosome aberration and the number of fragile sites expressed in each sample type and frequency of occurrence. Results The types and the aberration rates of lymphocyte chromosome aberration in uterine leiomyoma were significantly higher than those in control group (P <0.01). Under the condition of APC culture, the expression rate of the fragile loci in the lymphocytes of patients was 70.2%, which was significantly higher than that of the control group (39.8%, P <0.01), and 20 kinds of fragile sites Point in more than 1/3 of the individual expression, only 8 kinds of control group, but also distribution characteristics are not the same. Conclusion Uterine leiomyoma patients with peripheral blood lymphocyte chromosome instability increased significantly.