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应用的间日疟原虫抗原(PvAg) 取自现症患者血中红内期裂殖体经分离冷冻制成;实验组系从外省感染间日疟后回当地接受氯喹加伯喹治愈的对象,在4 ~12 个月内接受采血,经淋巴细胞转化试验测定同位素标记的3HTdR放射性脉冲数,并计算出其几何均数、刺激指数,用t 分布进行统计学检验;用间接荧光抗体试验(IFAT)测定血清中的疟疾抗体水平;以无疟疾感染史的健康者做对照。结果PvAg 对疟疾免疫组T 淋巴细胞增殖反应高于对照组;植物血凝素(PHA) 的刺激反应在两组中无显著差别,而刺激指数前者高于后者;治愈后的疟疾患者均产生了疟疾抗体,抗体几何平均滴度为1∶47 .24 。表明间日疟患者治愈后T 淋巴细胞在体外对PvAg 刺激呈现较强的增殖反应;抗体滴度随时间延长而降低。
The applied Plasmodium vivax antigen (PvAg) was obtained from frozen-thawed schizonts in the blood of patients with pre-existing disease. The experimental group was treated with chloroquinc or gabapentin to recover from Plasmodium vivax in other provinces after infection. In 4 to 12 months to accept blood collection, measured by lymphocyte transformation isotope labeled 3H TdR radioactive pulse number, and calculate the geometric mean, stimulated index, using t distribution for statistical tests; indirect fluorescent antibody test (IFAT) was used to measure the level of malaria antibody in serum. The healthy males with no history of malaria infection were taken as controls. Results The results showed that the proliferation of T lymphocytes induced by PvAg in malaria group was higher than that of the control group. Phosphatidylinositol (PHA) stimulating reaction had no significant difference between the two groups, while the stimulus index was higher than that of the latter. The cured malaria patients For malaria antibodies, the geometric mean antibody titer is 1:47. 24. The results showed that T lymphocytes in vitro showed a strong proliferative response to PvAg stimulation in vitro after vivax malaria patients were cured; antibody titers decreased with time.