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目的:了解广州市水产品副溶血性弧菌污染状况并应用脉冲场凝胶电泳(PFGE)技术对28株副溶血性弧菌菌株进行分子分型分析。方法:2005~2006年陆续从餐饮单位、肉菜综合市场和超级市场采集虾、蟹、鱼、贝壳和生吃料理等168份检材,采用MPN法定量检测副溶血性弧菌,参照GB/T4789.7-2003分离、鉴定副溶血性弧菌;并采用限制性内切酶NotI,对28株副溶血弧菌进行PFGE分子分型,用BioNumerics Version 4.0软件(复选Dice相关系数和UPGMA方法)分型图谱进行聚类分析。结果:168份水产品共检出副溶血性弧菌57份,检出率为33.93%,其中贝壳类的带菌率最高,为55.56%;虾蟹类、鱼类次之(两者之间的带菌率无显著性差异,P>0.05);28株副溶血弧菌分为27个不同的PFGE型,归为7个聚类群(Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ、Ⅶ群),各群间相似系数<77.4%。结论:广州市水产品副溶血性弧菌污染较严重,卫生部门应采取有效措施防止由水产品引起的副溶血性弧菌食源性疾病;PFGE分型结果与菌株的水产品种类、采样时间、采样地点没有关联,提示当时当地水产品中副溶血性弧菌不存在流行趋势。
Objective: To understand the status of aquatic Vibrio parahaemolyticus in Guangzhou and to analyze the molecular typing of 28 strains of Vibrio parahaemolyticus by pulsed-field gel electrophoresis (PFGE). Methods: From 2005 to 2006, 168 samples of shrimp, crab, fish, shellfish and raw food were collected from catering establishments, comprehensive markets of meat dishes and supermarkets, and MPN method was used to detect Vibrio parahaemolyticus. According to GB / T4789.7-2003 Isolation and identification of Vibrio parahaemolyticus; PFGE molecular typing of 28 isolates of Vibrio parahaemolyticus was performed using restriction endonuclease NotI. BioNumerics Version 4.0 software (check Dice correlation coefficient and UPGMA method ) Typing map for cluster analysis. Results: A total of 57 Vibrio parahaemolyticus were detected in 168 aquatic products, the detection rate was 33.93%, of which 55.56% was the highest in shellfish, followed by shrimp, crab and fish 28 isolates were divided into 27 different PFGE types and grouped into 7 clusters (Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ and Ⅶ) , The similarity coefficient of each group was <77.4%. Conclusion: The pollution of Vibrio parahaemolyticus in aquatic products is more serious in Guangzhou City. The health department should take effective measures to prevent the foodborne diseases caused by Vibrio parahaemolyticus caused by aquatic products. The results of PFGE typing and aquatic species, sampling time There was no correlation between sampling sites, suggesting that there was no epidemic tendency of Vibrio parahaemolyticus in local aquatic products at that time.