支气管哮喘多为过敏性哮喘,查明过敏原至关重要,目前常用的方法有体内诊断和体外诊断两类。体内诊断诸法皆有引起全身严重反应的危险,因此近年多重视体外诊断的研究,自七十年代后,国外建立了人类嗜碱细胞脱颗粒试验(Human basophil degranula-tion test HBDT),为变态反应的体外诊断开辟新途径,但该法用血量偏多(10～30ml),为临床普及应用带来一定困难。我们将该法略加改进,采血量3ml、将分离细胞的分层液比重改为1.087,巴比妥—EDTA 缓冲液的 pH 改为7.6,结果较为满意,现报告如下:材料和方法1.受检对象:共计43人,其中,确诊为过敏性哮喘者25人,另18人为皮试阴性的正常对照。所用抗原为吸入性抗原,由北京 Bronchial asthma is mostly allergic asthma, allergen identification is essential, the current methods commonly used in vivo diagnosis and in vitro diagnosis of two categories. In vivo diagnostic methods are all caused by the risk of serious systemic reactions, so in recent years, more emphasis on in vitro diagnostic studies since the seventies, the establishment of a foreign human basophil degranulation test (Human basophil degranula tion test HBDT) In vitro diagnostic reaction opens up a new way, but the law with excessive blood volume (10 ~ 30ml), for clinical application of certain difficulties. We will slightly improve the method, the amount of blood 3ml, the separation of cells to change the proportion of stratified liquid to 1.087, the pH of barbiturate-EDTA buffer to 7.6, the results are more satisfactory, are as follows: Materials and methods 1. Subjects: a total of 43 people, of which 25 were diagnosed as allergic asthma, and the other 18 were skin test negative for the normal control. Antigens used for inhaled antigen, from Beijing