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Objective To study the identification of the cause of specific sperm abnormalities.Methods Two adult men with specific alterations in sperm morphology causing 100%immobility were included in this study. The study of sperm used: transmission electron microscopy(both patients); apoptotic markers, DNA fragmentation test and fluorescence in-situ hybridization(patient 1) and immunocytochemistry study of sperm flagellum using anti-β tubulin antibodies and ciliary activity test(patient 2).Results Increased DNA fragmentation(52.6%) and apoptosis biomarkers were detected in patient 1, and loss of the central pair of microtubules in patient 2(‘9+0’axoneme); the nasal ciliary activity was normal.Conclusion Results suggest an apoptotic origin of the abnormalities in the sperm from patient 1 and dysplasia of the fibrous sheath in patient 2.
Objective To study the identification of the cause of specific sperm abnormalities. Methods Two adult men with specific alterations in sperm morphology causing 100% immobility were included in this study. The study of sperm used: transmission electron microscopy (both patients); apoptotic markers, DNA fragmentation test and fluorescence in-situ hybridization (patient 1) and immunocytochemistry study of sperm flagellum using anti-β tubulin antibodies and ciliary activity test (patient 2). Results Increased DNA fragmentation (52.6%) and apoptosis biomarkers were detected in patient 1 , and loss of the central pair of microtubules in patient 2 (’9 + 0’axoneme); the nasal ciliary activity was normal. Confclusion Results suggest an apoptotic origin of the abnormalities in the sperm from patient 1 and dysplasia of the fibrous sheath in patient 2.