论文部分内容阅读
目的:研究大黄灵脾颗粒剂对5/6肾切除慢性肾衰竭(CRF)大鼠残余肾功能的影响及其可能机制。方法:随机分为模型组、福辛普利组、大黄灵脾颗粒剂组和假手术正常组。除假手术组外余动物行5/6肾切除。第1次手术后,各组开始灌胃给药,直至第2次手术后8周结束。期间分时间点检测各组大鼠血清尿素氮(BUN)、血肌酐(SCr)、血管紧张素Ⅱ1型受体(AT1),观察大黄灵脾冲剂对肾衰大鼠肾功能、肾脏病理及免疫组化的影响。结果:大黄灵脾组大鼠的SCr在第4周开始出现下降,与模型组比较有显著性差异,与福辛普利组比较差异显著;在大鼠肾组织学变化上大黄灵脾组和福辛普利组的肾小球肥大情况较模型组有减轻,大黄灵脾组与模型组比较,差异显著,大黄灵脾组优于福辛普利组;AT1的表达上大黄灵脾组与模型组比较在6周有显著差异(P<0.05),而福辛普利组与模型组比较在3周就有显著差异。结论:大黄灵脾颗粒剂能够延缓大鼠慢性肾衰的进展,减轻肾脏的损害。
Objective: To investigate the effects of Dahuang Lingbi Granules on residual renal function in 5/6 nephrectomized chronic renal failure (CRF) rats and its possible mechanism. Methods: They were randomly divided into model group, fosinopril group, rhubarb lingzhi granule group and normal sham operation group. Except for the sham group, 5/6 nephrectomy was performed. After the first operation, each group began to be administered intragastrically until the end of the second operation 8 weeks later. Serum urea nitrogen (BUN), serum creatinine (SCr), and angiotensin II type 1 receptor (AT1) were measured at various time points in each group. The kidney function, renal pathology, and immunity of rats with renal failure were observed. The effect of grouping. RESULTS: The SCr of rats in the rhubarb spirit and spleen group began to decline at the 4th week, which was significantly different from that in the model group, and it was significantly different from that of the fosinopril group. The glomerular hypertrophy in the fosinopril group was less than that in the model group. The difference was significant between the rhubarb and spleen group and the model group. The rhubarb Lingpi group was superior to the fosinopril group; the expression of AT1 was related to the rheumatoid arthritis group. The model group was significantly different at 6 weeks (P<0.05), while the fosinopril group was significantly different from the model group at 3 weeks. Conclusion: Dahuang Lingpi granules can delay the progression of chronic renal failure in rats and reduce the damage of the kidney.