沒有分化出子叶的銀杏幼胚进行离体无菌培养,在含有无机盐,蔗糖,四种維生素及甘氨酸(在試驗5中并加入銀杏雌配子体提取液及0.1ppm IAA)的琼脂培养基中,不能进行正常分化。接种时已分化子叶的幼胚,在培养基上可以繼續生长或者引起愈伤組織似的生长。在基本培养基中加入蜂皇浆时,一部分未分化的幼胚可以得到正常分化发育,但小于1毫米的幼胚,仍不能生长,經过三个星期培养后即先后死亡。比較合适的蜂皇浆浓度是400ppm左右。看来蜂皇浆中含有某些营养及刺激生长的物质,这些物质对于銀杏幼胚的器官分化和正常发育起一定的作用。 Ginkgo biloba embryos that did not differentiate into cotyledons were subjected to in vitro aseptic culture in an agar medium containing inorganic salts, sucrose, four vitamins and glycine (in Experiment 5, ginkgo female gametophyte extract and 0.1 ppm IAA) Can not be normal differentiation. Immature embryos of differentiated cotyledons at the time of inoculation can continue to grow or cause callus-like growth on the medium. When the royal jelly is added to the minimal medium, a part of the undifferentiated immature embryos can get normal differentiation and development, but immature embryos smaller than 1 mm still can not grow and die after three weeks of culture. Proper royal jelly concentration is about 400ppm. It appears that royal jelly contains some nutrients and stimulate the growth of substances that play a role in organ differentiation and normal development of ginkgo immature embryos.