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[目的]菌株Bacillus methylotrophicus是一株对人参锈腐病菌(Cylindrocarpon destructans)有较强拮抗作用的细菌,为了有效防治人参锈腐病,进行了其产生抗菌蛋白的性质研究和抑菌效果试验。[方法]通过混菌法研究抗菌蛋白对紫外、热、酸碱、有机溶剂和蛋白酶的稳定性;采用生长速率法测定抗菌蛋白对锈腐病菌菌丝生长的抑制作用;平板对峙法测定其对锈腐病菌菌丝形态的影响;液培法测定其对锈腐病菌孢子萌发的抑制影响。[结果]抗菌蛋白具有很好的抗紫外线、耐热、耐酸碱和耐蛋白酶的稳定性,当质量浓度大于62.5 mg/L时,对人参锈腐病菌菌丝生长抑制率超过63.8%;显微镜观察其对菌丝生长有强烈的抑制作用,可以导致菌丝生长过程中分支增多、断裂和菌丝空洞,500 mg/L的抗菌蛋白对锈腐病菌孢子萌发的抑制率可达到100%。[结论]为人参锈腐病的防治提供新的生物防治资源。
[Objective] The bacterial strain Bacillus methylotrophicus was a bacterium with strong antagonism against Cylindrocarpon destructans. In order to effectively prevent and cure the rust of ginseng, the antibacterial properties and antibacterial activity of Bacillus methylotrophicus were studied. [Method] The stability of UV, heat, acid and alkali, organic solvent and protease of antibacterial protein were studied by the method of mixed culture. The inhibition of the growth of mycorrhizal fungi by antibacterial protein was determined by growth rate method. The influence of mycelium morphology on the pathogen of pathogen rust was tested by liquid culture method. [Result] The antibacterial protein had good anti-UV, heat, acid and alkali resistance and protease-resistant stability. When the concentration was over 62.5 mg / L, the mycelium growth inhibition rate of GTC was more than 63.8% It was observed that the mycelial growth was strongly inhibited, which led to the increase of branches, hypha and mycelium in the mycelium growth. The inhibition rate of 500 mg / L antimicrobial protein on the spore germination of the pathogen was up to 100%. [Conclusion] The article provided new biological control resources for the prevention and treatment of ginseng rust.