Objective To compare the roles of fluorescence in situ hybridization(FISH) and karyotype analysis on chorionic villus in spontaneous abortion.Methods A total of 201 cases were included in this study and were randomly divided into 2 groups by the same gestational age.The villi from 100 cases(group A) were cultured and used for karyotype analysis,while the uncultured villi from 101 women(group B) were used for FISH.A case was randomly selected from pregnant patients of6-11 weeks at each gestational week in each group,6 cases from each group and a total of 12 cases were analyzed by FISH and karyotype at the same time.Results The successful karyotype analysis rate was 66.0%,and the abnormality karyotype rate was 30.3%;FISH success analysis rate was 100%,and the abnormality rate was 46.5%;there were significant differences between FISH and karyotype analysis(P=0.036).Conclusion There were obvious differences between the two techniques.FISH was more successfully analyzed,and was used to more precisely determine fetal chromosome number.It is an effective way to determine abnormal chromosome by integrating FISH and karyotype analysis in spontaneous abortion. Objective To compare the roles of fluorescence in situ hybridization (FISH) and karyotype analysis on chorionic villus in spontaneous abortion. Methods A total of 201 cases were included in this study and were randomly divided into 2 groups by the same gestational age.The villi from 100 cases (group A) were cultured and used for karyotype analysis, while the uncultured villi from 101 women (group B) were used for FISH. A case was randomly selected from pregnant patients of 6-11 weeks at each gestational week in each group, 6 cases from each group and a total of 12 cases were analyzed by FISH and karyotype at the same time. Results The successful karyotype analysis rate was 66.0%, and the abnormality karyotype rate was 30.3%; FISH success analysis rate was 100%, and the abnormality rate was 46.5%; there were significant differences between FISH and karyotype analysis (P = 0.036) .Conclusion There were obvious differences between the two techniques. FISH was more successfully analyzed, and was used to more A positive chromosome by integrating FISH and karyotype analysis in spontaneous abortion.