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目的 利用线粒体细胞色素C氧化酶抑制剂叠氮钠 ,观察其对模型大鼠学习记忆能力的影响。方法 SD大鼠皮下埋植Alzet微泵 ,连续恒速给予叠氮钠共 30天 ,给药 1mg·kg-1·h-1和 2mg·kg-1·h-1两个剂量组。Morris水迷宫、避暗实验检测动物学习记忆能力 ,旷场分析检测空间探索能力及兴奋性 ,攀网实验检测肌力。结果 微泵恒速灌注叠氮钠导致模型大鼠水迷宫游出时间及距离延长、错误次数增加 ,避暗潜伏期缩短、错误次数增加 ,旷场分析中对外界的空间探索能力、适应性及兴奋性均降低 ,但肌力无明显异常。结论 线粒体细胞色素C氧化酶活性下降可以导致动物学习记忆能力下降 ,微泵恒速灌注叠氮钠大鼠可作为一种拟痴呆模型 ,应用于发病机理及药理学等方面的研究
Objective To investigate the effect of mitochondrial cytochrome c oxidase inhibitor sodium azide on learning and memory in model rats. Methods SD rats were subcutaneously implanted with Alzet micropumps and were given sodium azide for 30 days at constant speed. Two doses of 1 mg · kg-1 · h-1 and 2 mg · kg-1 · h-1 were administered to SD rats. Morris water maze, dark avoidance test animal learning and memory ability, open field analysis test space exploration ability and excitability, climbing net test muscle strength. Results The constant infusion of sodium azide by micropump resulted in prolonged swimming time and distance of water maze in model rats, increased number of errors, shortening of latency to avoid darkening, increase of error times, spatial exploration ability, adaptability and excitement to the outside world in open-field analysis Sex are lower, but no obvious muscle strength abnormalities. Conclusions The decrease of mitochondrial cytochrome C oxidase activity may lead to the decrease of learning and memory ability in animals, and the constant infusion of sodium azide by micropumps may be used as a model for dementia in the study of pathogenesis and pharmacology