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为研究恒河猴骨髓基质细胞 (BMSC)体外培养及诱导分化为神经干细胞的可行性 ,分离恒河猴BMSC ,在体外应用神经干细胞培养液和细胞因子进行诱导分化 ,利用免疫细胞化学方法进行鉴定。结果发现 ,恒河猴BMSC能在体外培养中增殖、分化和表达干细胞特异性抗原神经巢蛋白 (Nestin) ,并最终能分化为胶质细胞样细胞和神经元样细胞 ;免疫细胞化学检测可见有神经元特异性烯醇化酶(NSE)和胶质原性纤维酸性蛋白 (GFAP)抗原表达 ;未发现维甲酸 (RA)、表皮生长因子 (EGF)和碱性成纤维细胞生长因子 (bFGF)对骨髓基质干细胞的诱导分化具有明显影响作用。提示恒河猴BMSC是具有较强的自我更新能力和多分化潜能的细胞 ,在一定条件下 ,可分化为表达神经系细胞 (神经元和神经胶质细胞)抗原的细胞 ,可作为神经细胞的种子细胞。
In order to study the feasibility of culturing rhesus monkey bone marrow stromal cells (BMSC) in vitro and inducing differentiation into neural stem cells, Rhesus monkey BMSCs were isolated and induced by neural stem cell culture medium and cytokines in vitro and identified by immunocytochemistry . The results showed that Rhesus monkey BMSC can proliferate, differentiate and express stemin-specific antigen Nestin in vitro and eventually differentiate into glial-like cells and neuron-like cells. Immunocytochemistry showed that there was Neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) antigen expression; no evidence of a combination of RA, epidermal growth factor (EGF) and basic fibroblast growth factor Bone marrow stromal cells induced differentiation has a significant effect. Suggesting that rhesus monkey BMSC is a cell with strong ability of self-renewal and differentiation, under certain conditions, can differentiate into cells expressing neuronal cells (neurons and glial cells) antigens, can be used as nerve cells Seed cells.